Figure 1.

MMPs are overexpressed in CRC and patient-derived PC: (A,B) RNA was isolated from FFPE sections of (A) primary colorectal tumours (n = 8) or (B) PC (n = 3) and adjacent healthy tissue and analysed for MMP2 (dots) and MMP9 (triangles) gene expression using RT-qPCR; two-sided unpaired t-test relative to healthy colon/peritoneum; * p < 0.05, ** p < 0.01, n.s. not significant. (C) FFPE sections (3 µm) of PC or adjacent healthy tissue were stained with antibodies against MMP9 or MMP2. The tissue of PC was strongly positive for MMP9 and weakly positive for MMP2. Healthy tissue was negative for MMP9 and MMP2; Scale bar 200 µm.