Figure 2.

The experimental approach to classify viable or dead cells. (a) Schematic representation of cell seeding. (b) The clusters of cells marked with a yellow circle were excluded from the analysis. Cells marked with a blue arrow (dark blue arrow, C1; or light blue arrow, C2) were recorded at t = 24 h and t = 36 h for comparative analysis, to identify proliferating cells. These cells were classified as viable cells. (c) Cells marked with red arrows (light red arrow, C3; or dark red arrow, C4) were recorded at t = 56 h and t = 72 h for comparative analysis, to identify lysed cells. These cells were classified as dead cells.