Figure 3.

Correlation between FV fluorescence intensity, and cell viability or death. (a) FV intensity values measured in viable cells. (b) FV intensity values measured in dead cells. (c) Cell C1 replicated at t = 36 h, and its daughter cell, C1.1, at 80 h. The FV intensity values of the C1 cell for up to 36 h were recorded as viable FV intensity values. Similarly, all FV intensity values of the C1.1 cell up to 80 h were accounted for as viable FV intensities. The C1.2 cell did not replicate, so its FV intensity values were discarded (black crosses). Cell C2 replicated at t = 36 h. After this replication, none of the daughter cells replicated. Therefore, all FV intensity values up to 36 h are accounted for as viable FV intensity values, and the rest are discarded (black crosses). All viable FV intensities are considered to form the viable FV intensity range (within the dashed blue lines) (15 values in this example). (d) Both cells (C3 and C4) were lysed between the 56 and 72 h measurements. Therefore, the measurements at t = 0 h and t = 9 h are discarded (black crosses), as they are not within the 48 h span. (e) All viable intensities of FV recorded during the 80 h monitoring period constitute the “live cell sample” (15 values in the example). (f) All intensities of FV recorded during the 80 h monitoring period that are associated with dead cells constitute the “dead cell sample”.