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. 2022 Jul 28;14(15):3676. doi: 10.3390/cancers14153676

Figure 7.

Figure 7

Genotoxin screening for PARylation using the WWE split luciferase assay. U2OS cells expressing WWE-nLuc (or WWE(R163A)-nLuc) and WWE-cLuc were treated with individual compounds at the specified concentration. The vertical dotted line denotes a DMSO-normalized value. (A) PAR formation following 30 min of compound exposure. Values are normalized to untreated controls. (B) PAR formation following 6 h of compound exposure. For (A,B), each dot represents a single plate reading, with mean and SEM (red bars) shown; N ≥ 8 reads. * p < 0.05 using a two-sample t-test in comparison between WWE-nLuc and WWE(R163A)-nLuc for each test condition.