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. 2022 Aug 2;14(15):3763. doi: 10.3390/cancers14153763

Figure 2.

Figure 2

LIPG knockdown inhibits OCR. (A,B) Oleic acid uptake assay in MDA-MB-468 cells (WT), and LIPG or DANCR shRNA stable MDA-MB-468 cells. (C,D) Seahorse analysis of oxygen consumption rate (OCR) in wild-type (WT) and LIPG/DANCR siRNA knockdown MDA-MB-468 cells. OCR was measured, and the following drugs were used for the assay: oligomycin (oligo, 0.5 µg/mL); carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP, 4 µM), and antimycin (AA, 1 µM). (E,F) Seahorse analysis of OCR in wild-type (WT) and LIPG overexpression (OE) MCF-7 or shRNA knockdown (KD) MDA-MB-468 cells. (G,H) Tumor cells, as indicated, were treated with or without 150 µM FFA, and OCR was subsequently analyzed.