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. 2022 Jul 29;11(15):2341. doi: 10.3390/cells11152341

Figure 2.

Figure 2

Effect of combined heat and osmotic stress on cell wall suberization of Arabidopsis primary root (PR), at secondary (a,d) and primary (c) development stages, and lateral roots (LR; b,e). Expression of suberin biosynthetic gene GPAT5 was detected after 24 h of control and combined heat and osmotic stress treatments using the promoter line pGPAT5::mCITRINE-SYP122 (a–c). FY staining was used in col-0 Arabidopsis to detect suberin after 24 h of control and combined heat and osmotic stress treatments (d,e). Arrowheads indicate GPAT5 expression and FY staining in cortex cells of LRs. The 3D maximum projection figures were obtained by confocal laser scanning with Z-stack images. Pixel values were converted in a thermal look-up-table. Scale bar-50 µm.