Figure 4.

Expression of selected genes involved in suberization and/or response to stress, in roots of Arabidopsis plants submitted to combined heat and osmotic stress (gray bars) as compared to control (white bars) treatments. Root zones A (a) and B (b) were analyzed individually at selected time points from 0 h (before stress imposition) to 24 h after stress initiation. Gene expression was analyzed by RT-qPCR and normalized with the housekeeping gene UBQ21. Mean expression ± standard deviation from 3 independent experiments is represented for each time point. Asterisks (*) represent significant differences (p < 0.05, unpaired t-test) between control and combined heat and osmotic stress for a given time point.