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. 2022 Jul 30;14(15):3726. doi: 10.3390/cancers14153726

Figure 3.

Figure 3

Src knockdown and inhibition in OAC cells. (a) Western blot analysis of basal phospho-Src Y416 and total Src protein in OAC cells with β-actin as a loading control. Src knockdown with 10 nM SRC-targeting siRNAs (SRC-I and SRC-II) for 72 h validated by (b) Western blot of protein levels with vinculin as a loading control and (c) Q-PCR of gene expression levels. NC, negative control siRNA. Western blot analysis of phospho-Src Y416 following 24 h treatment with (d) dasatinib or (e) saracatinib in OE33, FLO-1, and MFD1 cell lines with vinculin as a loading control. (f) Western blot analysis of phospho-Src Y416 and total Src in MFD-1 cells transfected with (+) 10 nM siSRC or (−) 10 nM siNC for 24 h prior to 48 h CDDP (1 µM) treatment with vinculin as a loading control.