Skip to main content
. 2022 Aug 5;14(15):3810. doi: 10.3390/cancers14153810

Figure 1.

Figure 1

Altered ECM protein content in iCAFs-derived stroma. HFs and iCAFs were cultured in order to produce ECM sheets. Three cell sheets were stacked together, and urothelial cells were seeded on top to create 3D vesical models. (A) Young’s elastic modulus computed from tensile deformation test of HFs and iCAFs-derived stroma. N = 6 independent biological replicates. An unpaired t-test was performed. (B) Tile images of histological sections from HFs and iCAFs-derived models stained with Picrosirius red showing the collagen fibers. (C) Corresponding quantification of collagen content (N = 3 independent biological replicates). (D) Representative confocal images of HFs-derived and iCAFs-derived constructs stained for fibronectin (FN). (E) Corresponding quantification of FN signal based on the background normalized intensity (N = 3 independent biological replicates, 15 images per condition). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01.