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. Author manuscript; available in PMC: 2022 Aug 11.
Published in final edited form as: J Histotechnol. 2021 Aug 12;45(1):29–35. doi: 10.1080/01478885.2021.1951053

Table 2.

Decalcified ovine bone processing schedule on Sakura VIP E300.

Solution Number of Changes Time per change Temperature (°C) P/V Agitation
70% ETOH 1 4 h 37°C On
80% ETOH 2 4 h 37°C On
95% ETOH 2 4 h 37°C On
100% ETOH 3 4 h 37°C On
Xylene 2 4 h 37°C On
Paraffin 4 4 h 60°C On

ETOH: ethyl alcohol. P pressure; V vacuum.