Table 2.
Decalcified ovine bone processing schedule on Sakura VIP E300.
Solution | Number of Changes | Time per change | Temperature (°C) | P/V Agitation |
---|---|---|---|---|
70% ETOH | 1 | 4 h | 37°C | On |
80% ETOH | 2 | 4 h | 37°C | On |
95% ETOH | 2 | 4 h | 37°C | On |
100% ETOH | 3 | 4 h | 37°C | On |
Xylene | 2 | 4 h | 37°C | On |
Paraffin | 4 | 4 h | 60°C | On |
ETOH: ethyl alcohol. P pressure; V vacuum.