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. 2022 Jul 28;13:862757. doi: 10.3389/fimmu.2022.862757

Figure 5.

Figure 5

Fc-receptor profile of MLR cultures. PBMC from six healthy donors were co-cultured as three unique donor pairs to induce a mixed-leukocyte reaction (MLR), treated with anti-VISTA-antibody J014, and analysed for cell population frequency and Fc-receptor expression profile at the end of MLR culture. (A) Impact of anti-VISTA-antibody J014 on IgG4-Pro or IgG1-KO backbone on TNF levels in the MLR. Data is from one experiment; each single connected symbol represents a donor pair. (B) shows the frequency of major immune cell populations, (C) the frequency of Fc-receptor-positive cells within the identified immune cell populations, and (D) the Fc-receptor expression levels as median fluorescence intensity (MFI) of Fc-receptor-positive cells in MLR cultures either untreated (“none”) or treated with J014 on IgG4-Pro or IgG1-WT. Data are shown as mean values of three different donor pairs. Statistical significant differences between J014 antibody and its respective backbone isotype are indicated by a surrounding box. * indicates p < 0.05.