Figure 7.

anti-VISTA antibodies effects on resting immune cells require Fc-receptor interaction. PBMCs of single donors were cultured without stimulation and treated with anti-VISTA-antibodies. (A) Impact of different isotype backbones (IgG1-WT, IgG1-KO, IgG4-Pro) on the effects of anti-VISTA-antibody clones (VSTB112, E008, J014) on HLA-DR expression levels on monocytes. Data is from multiple experiments, each connected symbol represents a separate donor; the data for VISTA antibodies with IgG1-WT backbone is also shown in (Figure 4A). (B) Panel of cytokines and chemokines in cultures treated with anti-VISTA-antibody VSTB112 on three different isotype backbones (IgG1-WT, IgG1-KO, IgG4-Pro). Data is from one experiment with four unique donors; shown is the median fold-change of cytokine levels between VSTB112 on each backbone and the respective backbone isotype control. The data of IgG1-WT has also been included in Figure 6B. (C) Impact of blocking Fc-receptors on the effects of anti-VISTA-antibody J014-IgG1-WT on HLA-DR expression levels on monocytes. Data is combined from multiple experiments, each connected symbol represents a separate donor; the data for J014 with IgG1-WT backbone without blocking is also shown in (Figure 4A). * indicates p < 0.05.