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. 2022 Jul 28;13:862757. doi: 10.3389/fimmu.2022.862757

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Copyright © 2022 Mostböck, Wu, Fenn, Riegler, Strahlhofer, Huang, Hansen, Kroe-Barrett, Tirapu and Vogt

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of anti-VISTA antibodies in AML patient samples ex vivo. Bone marrow samples or PBMCs of AML patients were incubated ex vivo without stimulation, treated with various anti-VISTA-antibodies, and analysed by Pharmacoscopy. (A) Impact of anti-VISTA-antibody J014-IgG4-Pro on the interaction of CD3+ cells with CD34+ cells (left panel). Each symbol and color represents a distinct AML sample assayed in a separate experiment, described in the legend. Mean and SD shown for at least 3 technical replicates per sample and stimulation. (B) Mean pixel intensity of degranulation marker CD107a on CD3+ cell surface of a representative AML sample (AML #9 at 96h incubation time). Each symbol represents a technical replicate. (C) Impact of different isotype backbones (IgG4-Pro, IgG1-KO) on the effects of anti-VISTA-antibody J014 on the interaction of CD3+ cells with CD34+ cells. Each symbol and color indicates an AML sample assayed in a separate experiment, at 96h incubation time, see legend. Data of J014 on IgG4-Pro in (C) are also shown in (A, left panel). Mean and SD shown for at least 3 technical replicates per sample and simulation.** indicates p < 0.01, *** indicates p < 0.001, ns = "not significant".