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. 2022 Aug 8;11(1):487–500. doi: 10.1080/21623945.2022.2108558

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Exosomal miR-425-3p inhibited preadipocyte proliferation and differentiation. HPA-v cells were incubated with 50 μg/L of exosomes or 50 nM of miR-425-3p mimics for 24 h (for cell proliferation) or 7 days (for adipogenic differentiation), respectively. Adipocyte differentiation was induced using a standard protocol. CytoD (2 μg/mL) was administrated to inhibit endocytosis. Oil red O staining and western blot were performed at day 7 of adipogenic differentiation. (a) Effect of exosomes on cell proliferation. (b) Effect of exosomes on oil red O staining. (c) Effect of exosomes on the protein levels of aP2 and ADPN. (d) Effect of exosomes with miR-425-3p inhibition on cell proliferation. (e) Effect of exosomes with miR-425-3p inhibition on oil red O staining. (f) Effect of exosomes with miR-425-3p inhibition on the protein levels of aP2 and ADPN. (g) Effect of miR-425-3p mimics on cell proliferation. (h) Effect of miR-425-3p mimics on oil red O staining. (i) Effect of miR-425-3p mimics on the protein levels of aP2 and ADPN. Data are presented as mean ± SD, n = 4; **p < 0.01, ***p < 0.001 vs indicated group. N-Exo: normal (NL20) cell-derived exosomes; C-Exo: cancer (A549) cell-derived exosomes; Mir-Inh-C-Exo: cancer (A549) cell-derived exosomes with miR-425-3p inhibition; CytoD: cytochalasin D; aP2: adipocyte protein 2; ADPN: adiponectin; CNTL: control.