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. 2022 Aug 8;11(1):487–500. doi: 10.1080/21623945.2022.2108558

Figure 6.

Figure 6.

MiR-425-3p targeted phosphodiesterase 4B (PDE4B). (a) The predicted miR-425-3p binding site in the 3ʹUTR of the PDE4B gene. (b) Luciferase reporter test of miR-425-3p and PDE4B. To observe the potential effect of exosomal miR-425-3p on PDE4B protein levels, mature adipocytes were incubated with 50 μg/L of exosomes or 50 nM of miR-425-3p mimics for 24 h. CytoD (2 μg/mL) was administrated to inhibit endocytosis. (c) Effect of exosomes on the PDE4B protein levels. (d) Effect of miR-425-3p mimics on PDE4B protein levels. Data are presented as mean ± SD, n = 3; **p < 0.01, ***p < 0.001 vs indicated group. N-Exo: normal (NL20) cell-derived exosomes; C-Exo: cancer (A549) cell-derived exosomes; CytoD: cytochalasin D; CNTL: control; WT: wild type; Mut: mutant.