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. 2022 Aug 8;11(1):487–500. doi: 10.1080/21623945.2022.2108558

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — PDE4B depletion did not affect preadipocyte proliferation and differentiation, but promoted adipocyte lipolysis and white adipocyte browning. HPA-v cells were infected with lentiviruses carrying PDE4B shRAN to reduce PDE4B expression. Adipocyte differentiation was induced using a standard protocol. Oil red O staining and western blot were performed at day 7 of adipogenic differentiation. (a) Cell proliferation. (b) The protein levels of GATA2, IGFBP4, and MMP4 in PDE4B KD or control HPA-v cells. (c) Oil red O staining. (d) The protein levels of aP2 and ADPN in differentiated cells. To observe the potential effects of PDE4B on adipocyte lipolysis and white adipocyte browning, lentiviruses carrying PDE4B shRAN were used to knockdown PDE4B in mature adipocyte. (e) Oil red O staining. (f) Glycerol concentration in culture medium. (g) UCP1 protein levels. Data are presented as mean ± SD, n = 4; **p < 0.01, ***p < 0.001 vs indicated group. KD: knockdown; aP2: adipocyte protein 2; ADPN: adiponectin; CNTL: control, ns: no significant.