Figure 1.

Treatment with Wnt3a leads to β-catenin nuclear translocation and transcriptional activity. Quantification of the transcriptional activity of β-catenin in CRC cell lines HT-29 (a) and HCT-116 (b). Cells were treated as indicated and for 24 h and the transcriptional activity was normalized to renilla quantification. Data are presented as the mean ± SEM of three independent experiments. Significance was determined using ANOVA followed by Bonferroni’s post-hoc test (*p < .05, **p < .01, and ***p < .001). Wnt3a affects the subcellular localization of β-catenin in CRC cells (c and d). Immunofluorescence analysis of β-catenin (green) localization. Nuclei were stained using DAPI (blue). White arrows indicate β-catenin spots in the nuclei. The fluorescence intensity was quantified using ICY Bioimage Analysis software (Institut Pasteur, Paris, France). Data are presented as the mean ± SEM of three independent experiments. Significance was determined using ANOVA followed by Bonferroni’s post-hoc test (**p < .01). Scalebar equivalent to 20 μm.