Table 1.
Showing studies on neutrophil in murine malaria.
| Mouse strain | Parasite strain/Load/route of infection | Procedure for neutrophil depletion | Strategy of neutrophil detection | Outcome of neutrophil depletion | Ref. |
|---|---|---|---|---|---|
| C57BL/6 | Transgenic P.berghei ANKA expressing ovalbumin (PbTg) infected RBC/5*104/i.v | 250 μg of anti-GR1(day 0 of infection or day 3-5 p.i for 30 mins i. p1) | Flow cytometry on blood samples: CD11b+ Ly6Cint Ly6G+ | CM developed on day 6 post infection, survival rate was 80% and no effect on parasitemia level. | (74) |
| C57BL/6 | Transgenic P.berghei ANKA expressing ovalbumin (PbTg) infected RBC/5*104/i.v. | 250 μg of anti-GR1(day 3 and day 5 i.p) | Flow cytometry on blood samples: CD45+ Ly6Cint Ly6G+ | Decreased CM development on day 6 p.i, high survival rate and no effect on parasitemia level. | (74) |
| C57BL/6 | P.berghei -ANKA infected RBC/1*106/i.p. | 200 μg of anti-CXCL10 (between day 3 and 9 p.i) | Flow cytometry on spleen samples: Ly6G+ | High CM, low survival rate and low parasitemia level. | (68) |
| C57BL/6 | P.berghei -ANKA infected RBC/1*105/i.v. | Pulmozyme (5mg/kg for every 8h i.p.) | Not Applicable | Low survival rate at 20%, 100% mortality by day 10 post infection and parasitemia increased day 6 p.i. | (49) |
| C57BL/6, BALB/c |
P.berghei -ANKA and NK65 infected RBC/1*106/i.p. | Immunization with rPbTatD or rPcTatD (50 μg intra-muscularly every 14 days). | Not Applicable | 100% survival rate and reduced parasitemia level. | (52) |
| CBA/NSlc | P.berghei -ANKA infected RBC/1*106/i.p. | 250 μg of anti-GR1 (day 1 or day 5 i.p.) | Tail blood | no CM development (low hemorrhage), survival rate was 90% by day 10 post infection and no effect on parasitemia level. | (67) |
| DBA/2 | P.berghei -ANKA infected RBC/1*106/NA | anti-GR1(0.2mg on day 1 p.i) | Flow cytometry on blood samples and microscopy of blood smear | Low MA-ARDS development, survival rate was 90% by day 10 post infection and no effect on parasitemia level. | (17) |
| 129/Ola + C57BL/6J mice | P.berghei -ANKA infected RBC/1*107/NA | anti-GR1(1mg on day 6 post infection i.p.) | Flow cytometry on blood samples: GR1+ | There was 60%-80% of CM development and survival rate was 0% after 24h of CM development. | (75) |
| CBA/Ca | P.berghei -ANKA infected RBC/1*106/i.p. | anti-GR1(0.5mg on day 5 post infection i.p.) | Microscopy of blood smear | BBB was not disrupted, delayed death and high survival rate (80%). | (76) |
| BALB/c | PyMDR/NA | anti-GR1(day 3 until day 7 post infection i.p.) | Not Applicable | Low liver injury (low AST, ALT and ALP levels2). | (77) |
| C57BL/6 | P.chabaudi AS infected RBC/1*104/i.v. | DNase-/- | Flow cytometry on blood samples: CD45+ CD3− Ly6G/Chi | Low liver injury, low AST level and no effect on parasitemia level. | (53) |
| C57BL/6 | P.chabaudi AS infected RBC/1*104/i.v. | NE/PR3−/− | Flow Cytometry on blood samples: CD45+ CD3− Ly6G/Chi | Low liver injury, low AST level and no effect on parasitemia level. | (53) |
| C57BL/6 | P.chabaudi AS infected RBC/1*104/i.v. | Anti-G-CSF (day 7 post infection i.v.) | Flow Cytometry on blood samples: CD45+ CD3− Ly6G/Chi | Low liver injury, low AST level. | (53) |
| C57BL/6 | P. yoelli 17XNL infected RBC/2*104/i.v. | MPO−/− | Not Applicable | Not determine, however no effect on parasitemia level on day 6-12 post infection, parasitemia level increased after day 12 post infection. | (78) |
| C57BL/6 + Py17XNL | P. yoelli 17XNL infected RBC/2*104/i.v. | Anti-Ly6G (500 μg i.p.) | Flow Cytometry on blood samples: CD11b+ Ly6Cint GR1+ | Not determine, however no effect on parasitemia level. | (78) |
i.p., Intraperitoneal; i.v., Intravenously; p.i, Post infection; i.m., Intramuscularly; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase.