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Purification and primary structural characterization of GFPA. GFPA was purified using (A) a DEAE-52 anion-exchange column and (B) a Chromdex 200 prep grade column. GFPA characterization and glycosyl composition were determined based on (C) UV spectra, (D) monosaccharide component chromatograms, (E) homogeneity and molecular weight, (F) molecular configuration analysis and (G) Fourier-transform infrared spectroscopy.
