FIG. 1.

Organization of the bop gene region and promoter mutagenesis. (A) Organization of the bop gene region (bop, brp, bat, and blp genes) shown in boxes with the promoters indicated above by arrows. (B) Minimum bop promoter and a few surrounding nucleotides. The sequence is numbered starting with the transcription start point (indicated by an arrow) as +1. The UAS, TATA box, and R-Y box regions are boxed, and the translational start codon is underlined. The locations of osmium tetroxide and S1 nuclease cleavages are indicated by vertical arrows (36). (C) E. coli-Halobacterium shuttle vector (pNB series), which contains the bop gene (black arrow) and promoter (white box). The E. coli replication origin (ori ColE1) and selectable marker (bla [shaded arrow]) are indicated, as are the Halobacterium replication origin (ori pNRC100), repH gene (white arrow), and selectable marker (mev [shaded arrow]).