Figure 7.

Denatonium influences cAMP-dependent ion currents. (A) Schematic drawing of the effect of pertussis toxin (PTX). (B) The inhibitor of the G_αi subunit pertussis toxin (500 ng/mL, apical) reduced the denatonium-induced current (∆I_SC, n = 5, * p < 0.05). (C) In the presence of the phosphodiesterase inhibitor IBMX (100 µM apical), the denatonium-induced effect was increased (∆I_SC, n = 5, * p < 0.05). (D) Apical application of 1 mM denatonium decreased I_SC, which was reversed by forskolin (50 µM, apical). Representative current trace. (E) Forskolin increased I_SC in the presence of denatonium by approximately the same extent as it was decreased before with denatonium (∆I_SC, n = 6).