Figure 3.
Cytoplasmic aggregates of truncated TDP-43 include K63- and M1-ubiquitins. (A) GFP and various A315T mutants of TDP-43-GFP were transiently expressed in Neuro2a cells, and the colocalization of K63-ubiquitin (K63-Ub) and linear ubiquitin (M1-Ub) was examined. (B) Similar experiments in (A) were performed after a treatment with 2 μM MG-132 for 6 h. Bars = 10 μm.
