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UV survival of BL21(DE3)/pLysS cells and derivatives. E. coli BL21(DE3)/pLysS (uvrD+) (●), BL21(DE3)ΔuvrD/pLysS (ΔuvrD) (○), BL21(DE3)ΔuvrD/pLysS/pET11d-UvrD (uvrD+) (■), BL21(DE3)ΔuvrD/pLysS/pET11d-UvrDΔ40C (uvrDΔ40C) (□), and BL21(DE3)ΔuvrD/pLysS/pET11d-UvrDΔ102C (uvrDΔ102C) (▴) were exposed to increasing doses of UV light as indicated; 100% survival was defined as the number of colonies present in an unirradiated sample. Data are averages of three individual experiments.
