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. 2022 Aug 2;11(15):2369. doi: 10.3390/cells11152369

Figure 4.

Figure 4

Effect of GPR17 silencing on OPC maturation. The expression levels of GPR17 (green), NG2 (blue) and MBP (red) were evaluated by real-time PCR during differentiation in control conditions (a), cells transfected with scramble RNA and after GPR17 silencing (b), and normalized versus expression at T0 (set to 0). n = 6 per each time point. One-way ANOVA with Tukey’s multiple comparisons test. (a): *** p < 0.001 vs. DID 0-2-3-5, @ p < 0.05 vs. DID 3-5; ## p < 0.01 vs. day 0; §§§ p < 0.001 vs. DID 0-1-2-3; (b): *** p < 0.001 vs. DID 0-2-3-5, @@@ p < 0.001 vs. day 0, # p < 0.05, ### p < 0.001 vs. day 0, § p < 0.05, §§ p < 0.01, §§§ p < 0.001 vs. DID 5. (c) NG2 and MBP expression in GPR17-silenced OPCs vs. control OPCs (set to 0) at each time point. Fold change (FC) has been reported as LOG2(FC). One sample t-test, * p < 0.05; ** p < 0.01; *** p < 0.001 vs. relative DID in control OPCs.