Table 1.

The in vitro effects of m⁶A modifications by methyltransferases METTL3/METTL14, “readers” YTHDF1/2/3, and demethylases ALKHB5/FTO on SARS-CoV-2 viral replication and protein expression.

m6A Modification Enzymes	Cell Line/Extraction Time	Method	Effects	Reference
METTL3	Caco-2/24 hpi	Knockdown	Decreased replication	[251]
METTL3/METTL14	Huh7/72 hpi	Knockdown	Increased replication	[802]
YTHDF2	Huh7/72 hpi	Knockdown	Increased replication	[802]
ALKHB5	Huh7/72 hpi	Knockdown	Decreased replication	[802]
FTO	Vero/14 hpi	Knockdown	Demethylase inhibitor dose-dependent interference with viral lifecyle with complete blockage of infection at highest dose.	[810]
METTL3	Vero/24 hpi	Knockdown	Decreased viral titer, N protein copy number and expression	[811]
METTL3	Vero/24 hpi	Overexpression	Elevated m6A modification	[811]
FTO	Vero/24 hpi	Knockdown	Increased viral titer, N protein copy number and expression	[811]
RBM15	HuT 78/24 hpi	Knockdown	Inhibited inflammatory gene expression, lymphocyte apoptosis	[812]
METTL3	A549+ACE/48 hpi	Knockdown	Reduced replication, synthesis of viral RNA and N protein	[813]
YTHDF1/YTHDF3	A549+ACE/48 hpi	Knockdown	Reduced replication, synthesis of viral RNA and N protein	[813]

hpi: hour post-infection.