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SLN1/sln1Δ tetrad dissections with various SLN1 expression constructs. (A) Full-length SLN1 with mutations incorporating restriction enzyme sites; (B) parental vector control; (C) SLN1KD (Δ1-438); (D) SLN1ΔKD (Δ543-1220). These results were obtained with both low- and high-copy-number SLN1-containing vectors.
