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Hog1p phosphorylation with Sln1p/leucine zipper constructions. (A) Antiphosphotyrosine immunoblot analysis of immunoprecipitated Hog1p. Lane 1, cells containing the ΔTMD1&ECD/LeuZip construct; lane 2, cells containing the ΔTMD1&ECD/LeuZip* (mutated, undimerizable leucine zipper) construct. (B) Duplicate immunoblot analysis of panel A with anti-Hog1p antisera showing similar amounts of Hog1p immunoprecipitated in all cases. The cells were grown in low-osmolarity medium.
