TABLE 3.
Comparison of group I deh genes and their expression in selected αHA-utilizing bacteria
| Organism (dehalogenase gene) | Group I deh PCR product (bp)a | RT-PCR product (bp)b | Presence of dehalogenase showing activity with d-2MCPAc |
|---|---|---|---|
| P. putida AJ1 (hadD) | 462 | NDd | + |
| Pseudomonas sp. strain 17a (dehI°17a) | 466 | 270 | − |
| Pseudomonas sp. strain 18a (dehI18a) | 460 | 400 | + |
| Pseudomonas sp. strain K55 (dehIK55) | 466 | 270 | + |
| Bradyrhizobium sp. strain DA2 (dehIDA2) | 466 | 330 | + |
| Strain DA1, β subclass of Proteobacteria (dehIDA1) | 466 | 400 | + |
| P. putida PP3 (dehIPP3) | 460 | ND | + |
| E. coli(pYW10) (dehI°PP3) | 469 | 290 | − |
a
Size determined by sequencing but not including the primer sequences.
b
RT-PCR was performed with specific primers as described in Materials and Methods.
c
As determined by gel zymography.
d
ND, not determined.