Figure 3.
Heterodimerization does not affect basal Ca2+ levels in N1E-115 cells. Neuroblastoma cells were transfected with Ca2+ indicator GCaMP6f, either alone or together with 5-HT2A-mTurquoise2, TrkB-YPet, or both. Basal Ca2+ activity was calculated as F/Fmax. To saturate Ca2+ levels, 10 µM ionomycin was applied after 6 min of recording. The saturated Ca2+ signal was used as Fmax. Data are presented as means ± SEM (12 ≤ n ≤ 17). *** p < 0.001 (Kruskal–Wallis test with Dunn’s multiple-comparison post hoc test).