FIG. 2.

Disappearance of the maltose transporter from the plasma membrane in a mutant defective in clathrin. Strains GPY1100 (CHC1, wild type [WT]) and GPY418 (chc1-1), transformed with the plasmid pRM1-1 carrying the MAL1 locus, were grown and treated as described in the legend for Fig. 1. After incubation at 24°C (A and C) or 35°C (B and D) maltose transporter (A and B) and H⁺-ATPase (C and D) were detected by immunoblotting aliquots containing 7 and 3 μg of protein, respectively, of purified plasma membrane preparations obtained at the indicated times.