Figure 6.

Recovered migratory activity of MNPs@SiO₂(RITC)-treated BV2 cells by citrate treatment. (A) Transwell assay of MNPs@SiO₂(RITC) and citrate co-treated BV2 cells. The nuclei of the cells were stained using Hoechst 33342, and the fluorescence was expressed as cyan. Fluorescence of MNPs@SiO₂(RITC) distribution was expressed as red. The cells were counted under a fluorescence microscope in five randomly selected fields. The number of nuclei was counted in five randomly selected fields. Scale bar = 50 μm. Scale bar of cropped image = 10 μm. (B) The number of migrated cells. Data represent means ± standard deviation of three independent experiments. * p < 0.05 vs. control. ** p < 0.01 vs. control. (C) Viability of MNPs@SiO₂(RITC)-treated BV2 cells in the presence of 1 mM citrate for 12 h. (D) Intracellular ATP levels in MNPs@SiO₂(RITC)-treated BV2 in the presence of 1 mM citrate for 12 h. Data represent means ± standard deviation of three independent experiments. * p < 0.05 vs. control.