TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| B. fragilis | ||
| TM4000 | Rifr | 15 |
| TM4.23 | Tetr Rifr | 15 |
| LV23 | Clinical isolate | |
| E. coli | ||
| HB101 | Smr | 31 |
| DW1030 | Spr | 31 |
| DH5α | 3 | |
| Plasmids | ||
| pBR328 | Mob− Cmr Tetr Apr | 9 |
| pOX38::Kan | Knr, 56-kb HindIII fragment of F plasmid | 18 |
| F′ lac | IncF1, Tra+lac+ | Pasteur Institute |
| pGAT400 | Clnr Apr Tetr (aerobic) Tra+ | 15 |
| pGAT400ΔBglII | Clnr Apr Tetr (aerobic) Tra− | 15 |
| pHB23Δ5 | Clnr Apr Tets (aerobic) Tra+ | This study |
| pKB1 | Apr, 3.7-kb fragment of Tn5520 with bmpH gene in pBR328 | This study |
| pTJ20 | Apr Tra+ | This study |
| pTJ22.3 | Tn1000 insertion in bipH of pTJ20 | This study |
| pTJ22.26 | Tn1000 insertion in bipH of pTJ20 | This study |
| pTJ22.20 | Tn1000 insertion in bipH of pTJ20 | This study |
| pTJ22.25 | Tn1000 insertion outside bipH of pTJ20 | This study |
| pTJ22.10 | Tn1000 insertion outside bipH of pTJ20 | This study |
| pTJ22.36 | Tn1000 insertion outside bipH of pTJ20 | This study |
| pTJ22.24 | Tn1000 insertion outside bipH of pTJ20 | This study |
| pTJ23 | Deletion of B end of Tn5520 | This study |
| pGV2 | Tn1000 insertion in bmpH of pTJ20 | This study |
| pGV3 | Tn1000 insertion in bmpH of pTJ20 | This study |
| pGV4 | Tn1000 insertion outside bmpH (proximal) of pTJ20 | This study |
| pGV5 | Tn1000 insertion outside bmpH (proximal) of pTJ20 | This study |
| pGV6 | Tn1000 insertion outside bmpH (proximal) of pTJ20 | This study |
| pGV7 | Tn1000 insertion outside bmpH (distal) of pTJ20 | This study |
| pGV8 | Tn1000 insertion outside bmpH (distal) of pTJ20 | This study |
| pGV9 | Tn1000 insertion outside bmpH (distal) of pTJ20 | This study |
a
Rifr, Tetr, Smr, Spr, Cmr, Knr, Clnr, and Apr, resistance to rifampin, tetracycline, streptomycin, spectinomycin, chloramphenicol, kanamycin, clindamycin, and ampicillin, respectively; Tra+ and Tra−, transfer proficiency and deficiency, respectively; Mob−, inability to be mobilized; Tets, tetracycline sensitivity.