TABLE 2.
Transfer of Tn5520 in B. fragilis and mobilization in E. coli
| Process | Donor strain | Plasmid | Frequency of transfer (mean ± SE)a |
|---|---|---|---|
| Plasmid transfer (B. fragilis to E. coli) | TM4000 | pGAT400 | (7.9 ± 3.8) × 10−9 |
| pGAT400ΔBglII | NDb | ||
| pHB23Δ5 | (4.5 ± 3.1) × 10−8 | ||
| TM4.23 | pGAT400 | (3.6 ± 1.6) × 10−5 | |
| pGAT400ΔBglII | ND | ||
| pHB23Δ5 | (1.5 ± 0.83) × 10−4 | ||
| R751 mobiliza-tion (E. coli to E. coli) | HB101(R751)c | pBR328 | ND |
| pGAT400 | (0.4 ± 0.17) × 10−2 | ||
| pTJ20 | (3.2 ± 0.05) × 10−1 | ||
| pKB1 | (0.3 ± 0.1) × 10−2 | ||
| pGV2d | ND | ||
| pGV3d | ND | ||
| pGV4e | (3.7 ± 2.9) × 10−7 | ||
| pGV5e | ND | ||
| pGV6e | (3.3 ± 1.5) × 10−6 | ||
| pGV7f | (1.6 ± 0.09) × 10−2 | ||
| pGV8f | (1.02 ± 0.095) × 10−2 | ||
| pGV9f | (1.4 ± 0.05) × 10−2 |
a
Experiments were performed at least in triplicate.
b
ND, transfer or mobilization not detectable (≤10−9 [Bacteroides] or no transconjugants on selective plates).
c
The IncPβ plasmid R751 was coresident in the donor during E. coli-to-E. coli mobilization experiments.
d
Tn1000 insertion in bmpH.
e
Tn1000 insertion proximal to bmpH.
f
Tn1000 insertion distal to bmpH.