TABLE 3.
Transposition of Tn5520 in E. coli
| Donor | Transposition frequency (mean ± SE)a |
|---|---|
| DW1030(pOX38::Kan, pTJ20)b | (1.1 ± 0.2) × 10−5 |
| DW1030(pOX38::Kan, pBR322)c | (7.7 ± 1.9) × 10−8 |
| pTJ22.23d | (1.9 ± 1.2) × 10−7 |
| pTJ22.26d | (1.2 ± 0.05) × 10−7 |
| pTJ22.20d | (1.2 ± 0.3) × 10−7 |
| pTJ22.25e | (7.5 ± 2.3) × 10−5 |
| pTJ22.10e | (2.9 ± 0.9) × 10−5 |
| pTJ22.36e | (1.9 ± 0.6) × 10−5 |
| pTJ22.24e | (5.1 ± 2.9) × 10−5 |
| pTJ23f | (7.7 ± 1.9) × 10−8 |
Experiments were performed at least in triplicate.
Positive control.
Negative control.
Mutant with Tn1000 insertion in bipH.
Mutant with Tn1000 insertion outside bipH.
Deletion of Tn5520 B end.