Figure 5.

Increased beating variability and arrhythmic events in BrS-hiPSC-CMs. Spontaneous calcium transients were recorded in spontaneously beating hiPSC-CMs from the BrS patient, the healthy donor (healthy) and the CRISPR-corrected cells (isogenic). The beating variability (standard deviation (SD) of cell beating intervals) and the occurrence of arrhythmic events (irregular or triggered beats or EAD-like events) were compared among the three cell groups. (A–C) Representative traces of calcium transients in cells from each line. Arrhythmic events are marked by arrows. (D) Median values of standard deviation (SD) of cell beating intervals. (E) Percentage of cells showing arrhythmic events. The numbers given represent number of cells showing arrhythmic events versus total cell number. The numbers given represent number of cells. * p < 0.05 versus healthy according to the one-way ANOVA (D) or Fisher’s exact test (E).