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. 2022 Jul 26;23(15):8226. doi: 10.3390/ijms23158226

Figure 2.

Figure 2

Expression of the stem cell and ocular biomarkers in limbal stem cells. Panel of the representative images of the limbal stem cells showing positive expression of ABCG2, ABCB5, P63-α, and PAX6 (red) in both epithelial (LMSC−P0) and stromal cell (LMSC−P3) populations, counterstained with DAPI (blue). Scale: 50 µm. Level of expression (lower panel) of ABCG2, ABCB5, P63α, and PAX6 genes quantified using qRT-PCR in limbal epithelial (LMSC−P0) and stromal (LMSC−P3) cells, relative to native limbal tissue (n = 5). P63α was found to be down-regulated in LMSC−P3 where all the other stem cell genes ABCG2, ABCB5 and PAX6 were found to follow same pattern of expression in both early and late passages of the culture. The results were plotted as mean log 2-fold change ± SD. The statistical analysis was performed using Kruskal–Wallis one-way ANOVA test. # p > 0.05, ** p < 0.01, *** p < 0.001.