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. 2022 Jul 26;23(15):8226. doi: 10.3390/ijms23158226

Figure 3.

Figure 3

Limbal stem cells showing mesenchymal stem cell biomarkers. Panel of the representative images of the limbal stem cells showing positive expression of Vim (Vimentin), CD90, and CD105 in both LMSC−P0 (n = 3) and LMSC−P3 (n = 3) populations, counterstained with DAPI (blue). Ncad (N-cadherin) were positive (red) in LMSC−P3 cells and Ecad (E-cadherin) did not show any expression in LMSC−P3. Level of expression of VIM, CD90, CD105, NCAD, ECAD genes quantified using qRT-PCR in LMSC−P0 and LMSC−P3 relative to native limbal tissue (n = 5). Except ECAD remaining genes were found to be up-regulated in LMSC−P3 with fold-change ranging between 2 to 20, which were down-regulated in LMSC−P0. Scale: 50 µm. The results were plotted as mean log 2-fold change ± SD. The statistical analysis was performed using Kruskal–Wallis one-way ANOVA test. * p < 0.05, *** p < 0.001.