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RNA primer extension analyses of V. cholerae strain O395 wild-type (A) and mutant strains toxTΔpro and tcpAΔpro (B). Samples were collected at 1-h intervals after back dilution of an overnight culture grown in LB medium at 30°C 1:100 into fresh LB medium. A radiolabeled tcpA primer was used. RNA collected from wild-type strain O395 after 2 to 3 h of growth was used as a positive control for the mutant strains in panel B. The position of the tcpA transcript is indicated.
