Figure 4.
Carfilzomib inhibits FOXM1 protein expression in AFP-positive cells. (A) FOXM1 and AFP expression in Huh7, MT, Kami41, and KM cells (n = 3 in each group, means ± SEM, one-way ANOVA test). (B) Western blot analysis of FOXM1 in AFP-positive and -negative cells. (C) Western blot analysis of FOXM1 in AFP-positive and -negative cells treated with carfilzomib for 24 h. Experiments were performed twice. (D) Viability of Huh7, MT, Kami41, and KM cells treated with carfilzomib for 24 h (n = 3 in each group, means ± SEM, Student’s t-tests, * p < 0.05). Experiments were performed twice.