Figure 2.

Knock down of Par4 in HCT116 cells. (A,B) Levels of Par4 mRNA. HCT116 cells were stably infected with shRNA-Par4. Par4 mRNA expression was assessed by RT-PCR, normalized to GAPDH (A) and by real-time PCR normalized to HPRT (B). (C,D) Tumor generation in wt and shRNA-Par4 HCT116 cells. Six-week-old male nude mice were injected subcutaneously with either wt HCT116 cells or shRNA-Par4 HCT116 cells (1 × 10⁶). The experiment was terminated after 5 weeks. These results are representative of the experiment performed three times. Number of mice per each treatment; n = 6 (2 died in the HCT116 wt-inoculated cells). Tumor volume (D) was measured as 0.5 (length) × width².