Figure 3.

Tumor generation by RKO/Par4 clones in nude mice. (A) Expression of Par4 mRNA in RKO and RKO/Par4 clone. RNA was isolated from the parental RKO cells and clones of RKO/Par4 (clones; a, b, c). Here, a representative of the RKO/Par4 clone is shown. Levels of Par4 were assessed by means of real-time qPCR normalized to HPRT. (B) Six-week-old male nude mice were injected subcutaneously with either wt RKO cells or RKO/Par4 clone cells (1.5 × 10⁶). The experiment was terminated after 5 weeks. These results are representative of the experiment performed with two repeititons. Number of mice /treatment n = 6 (3 RKO/Par4 died). RKO/Par4a (shown) and RKO/Par4b clones were inoculated. (C) Tumor volume was measured as 0.5 (length) × width². These results are representative of the experiment performed three times in mice.