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. 2022 Aug 6;23(15):8758. doi: 10.3390/ijms23158758

Figure 5.

Figure 5

The effect of GSK126 inhibitor on HT29 spheroids. (A) HT29 cells were grown in a Matrigel layer, supplemented with spheroid formation media. Small spheroids are seen in the presence of 2 μM and 52 μM GSK126, as compared with the wt HT-29-generated spheroids. Representative results of the experiment conducted four times are shown. (B) EZH2 level is increased following PAR4 activation. Western blot analyses of protein-lysates prepared from HT-29 spheroids before and after AYPGKF PAR4 activation. Increased levels of EZH2 are obtained, as per the β-Actin levels. (C) The effect of tcY-NH2, a PAR4 inhibitor in HT29 cells, on stem cell markers following AYPGKF PAR4 activation. qPCR analyses of a panel of stem cell markers in the presence and absence of the PAR4 inhibitor tcY-NH2 (100 μM). An upregulation was observed following AYPGKF PAR4 activation, inhibited in the presence of tcY-NH2.