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. 2022 Aug 6;23(15):8758. doi: 10.3390/ijms23158758

Table 1.

List of specific forward and reverse primers for each gene when qRT PCR was conducted.

Gene Forward Primer seq. Reverse Primer seq.
hprt 5′ACTGGCAAAACAATGCAGACTTT′3 5′GGTCCTTTTCACCAGCAAGCT′3
hPar4 5′CCCAGCGTCTACGACGAGA′3 5′CACAGACTTGGCCTGGGTAG′3
LGR5 5′CTTCCAACCTCAGCGTCTTC3′ 5′TTTCCCGCAAGACGTAACTC3′
CEACAM1 5′GCTTCTGCTCACAGCCTCAC3′ 5′CCTTCCCCTCTGCAACATTGA3′
CD44 5′CTGGGGACTCTGCCTCGT3′ 5′ACGTGGAATACACCTGCAAAGC3′
ALDH1 5′CACGCCAGACTTACCTGTCC3′ 5′TGCCACTCACTGAATCATGCC3′
OCT4 5′AGGAGAAGCTGGAGCAAAACC3′ 5′ATCCTCTTCTGCTTCAGGAGCT3′
OLFM1 5′GGCCAAGGTAGTGGTACAGC3′ 5′CTTGCCAAGCAACATTAGCA3′
CyclinD1 5′ACAAACAGATCATCCGCAAACAC3′ 5′TGTTGGGGCTCCTCAGGTTC3′