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. 2022 Aug 4;23(15):8676. doi: 10.3390/ijms23158676

Figure 3.

Figure 3

BIO promotes odonto/osteogenic differentiation but attenuates adipogenic differentiation of hDPSCs. (A) Osteogenic differentiation was determined by alkaline phosphatase (ALP) staining and mineral deposition. Scale bars: 300 µm. (B) Quantifying ARS staining. (C) The mRNA levels of osteogenic-related marker genes. (D) Timeline representing the pretreatment effect experiment. (E) Osteogenic differentiation was determined by mineral deposition. Scale bars: 300 µm. (F) Quantification of ARS staining. (G) Adipogenic differentiation was evaluated by intracellular lipid deposition. Scale bars: 30 µm. (H) The mRNA levels of adipogenic-related marker genes. Bars indicate a significant difference between groups (** p < 0.01. *** p < 0.001).