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. 2022 Jul 30;23(15):8454. doi: 10.3390/ijms23158454

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Single-dose application of the cobra venom factor (CVF (1x)) enhances the formation of platelet-neutrophil aggregates (PNAs) and the mobilization of neutrophils to the peripheral blood. Scatter plots show flow cytometry analyses of (a) the number of neutrophils per microliter of blood, (b) the number of platelets per microliter of blood measured by the differential blood count, and (c) the percentage of PNA formation relative to the total number of neutrophils in phosphate-buffered saline (PBS)- and CVF (1x)-treated mice 27 h after injection, respectively, 3 h after double femoral artery ligation (FAL) or a double sham operation (sham) of both femoral arteries. For the PNA analysis, platelets were detected by an anti-CD41 antibody, and neutrophils were identified by anti-CD11b and anti-Gr-1 antibodies. All values are the means ± S.E.M., n = 4 mice per group, * p < 0.05 by one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test.