Figure 3.

Scarb2^Adipoq-cre mice show lower lipid accumulation. (A) Schematic representation of the Scarb2 (top), and targeted gene (bottom). (B) The expression of Scarb2 in different tissues and organs from Scarb2^Adipoq-cre mice was analyzed by real-time quantitative PCR. Expression levels of target genes were normalized to Rplp0 (alias 36B4) and data presented in (B) were normalized to the mean value of WT group for each tissue or organ. n = 3. (C) Immunoblot analysis of Scarb2 and β-actin in heart, BAT, gWAT and scWAT from WT and Scarb2^Adipoq-cre mice. n = 4. (D,E) Body composition and percentage of fat mass of female WT and Scarb2^Adipoq-cre mice measured by DEXA after 12 weeks on a regular chow diet. n = 9. (F) HE staining of gWAT, scWAT and BAT sections from 12- week-old female WT and Scarb2^Adipoq-cre mice. Scale bars, 20 μm. (G) HE and Oil Red O staining of BAT sections from 12- week-old female WT and Scarb2^Adipoq-cre mice. Scale bars, 20 μm. DEXA: dual-energy X-ray absorptiometry, scWAT: subcutaneous white adipose tissue, gWAT: gonadal white adipose tissue, BAT: brown adipose tissue. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01.