Figure 2.

The shortest, standard isoform of CD44 (CD44s) is sufficient to confer resistance to doxorubicin (DOX). Osteosarcoma cells were seeded at 60% confluency in DMEM medium, supplemented with 7% FBS. The cells were incubated for 24 h in the presence of 1 µM DOX or pure solvent (control) and then subjected to immunoblot. The histograms in (A,B) show mean values of MDR1 or cleaved PAPR1 protein levels ± SD from three independent experiments. The values were normalized to loading control. Student’s t-test values: n.s.: p > 0.05, **: p ≤ 0.01, ***: p ≤ 0.001. Representative immunoblot is shown in (C).