Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 3;23(15):8616. doi: 10.3390/ijms23158616

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Gene expression analysis. (A) Principal component analysis (PCA) of RNA sequencing data. Data were plotted along the first and forth principal components. Each dot in PCA plot indicates a single sample. (B) Multivariate analysis of gene expression between Cd44-positive and Cd44-negative primary osteosarcomas. Unsupervised hierarchical clustering and heatmap showing Cd44+/+ and Cd44−/− primary osteosarcomas clearly segregated from one another, based on their RNA expression profiles. Three samples of each group were analyzed. Both downregulated (blue) and upregulated (red) RNAs were identified in Cd44−/− osteosarcomas. The analysis was performed using an MADE4 package of R software. (C) List of apoptosis-related genes that are differentially regulated between Cd44-positive and Cd44-negative osteosarcomas. The data are represented as Wald-statistic (stat). Genes with negative Wald-statistic values are down-regulated in Cd44−/− compared to Cd44+/+ primary osteosarcomas, and genes with positive values are upregulated. Benjamin Hochberg adjusted p-value (padj) is indicated. Gene description source: HGNC (www.genenames.org/, accessed on 31 May 2022).

Gene expression analysis. (A) Principal component analysis (PCA) of RNA sequencing data. Data were plotted along the first and forth principal components. Each dot in PCA plot indicates a single sample. (B) Multivariate analysis of gene expression between Cd44-positive and Cd44-negative primary osteosarcomas. Unsupervised hierarchical clustering and heatmap showing Cd44+/+ and Cd44−/− primary osteosarcomas clearly segregated from one another, based on their RNA expression profiles. Three samples of each group were analyzed. Both downregulated (blue) and upregulated (red) RNAs were identified in Cd44−/− osteosarcomas. The analysis was performed using an MADE4 package of R software. (C) List of apoptosis-related genes that are differentially regulated between Cd44-positive and Cd44-negative osteosarcomas. The data are represented as Wald-statistic (stat). Genes with negative Wald-statistic values are down-regulated in Cd44−/− compared to Cd44+/+ primary osteosarcomas, and genes with positive values are upregulated. Benjamin Hochberg adjusted p-value (padj) is indicated. Gene description source: HGNC (www.genenames.org/, accessed on 31 May 2022).