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. 2022 Aug 3;23(15):8616. doi: 10.3390/ijms23158616

Figure 6.

Figure 6

Testing mRNA levels of selected apoptosis-associated genes. Cd44-positive wild-type cells, Cd44-negative cells, and cells with reconstituted expression of Cd44s and Cd44ICD were seeded at 60% confluency in DMEM medium supplemented with 7% FBS. The cells were incubated for 24 h with pure solvent (controls) or 1.5 µM DOX. The histograms show mean values of mRNA levels ± SD from three independent experiments. Student’s t-test values: n.s.: p > 0.05, *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001, ****: p ≤ 0.0001.