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. 2022 Aug 1;23(15):8538. doi: 10.3390/ijms23158538

Figure 1.

Figure 1

Experimental protocol and diarrhea occurrence. (A) The mice were intraperitoneally sensitized on days 0 and 14, and intragastrically challenged using OVA on days 30, 32, 35, 37, and 39. From day 32 to day 39, mice were subjected to WAS or SS for 1 h before OVA or PBS challenge for eight consecutive days. In OVA+WAS-treated mice, CRH-R1 antagonist or vehicle were intraperitoneally administered 1.5 h before each OVA challenge. The mice were sacrificed 1 h after the final OVA challenge, and their ileums were analyzed. (B) The comparison of diarrhea occurrence among the four groups of mice: SS + PBS, SS + OVA, WAS + PBS, and WAS + OVA. * p < 0.05 and ** p < 0.01 between SS + PBS and SS + OVA, † p < 0.05 and †† p < 0.01 between SS + OVA and WAS + OVA. CRH-R1, corticotropin-releasing hormone type 1 receptor; OVA, ovalbumin; PBS, phosphate buffered saline; SS, sham stress; WAS, water avoidance stress.